This annex gives information on administering, reading and interpreting a tuberculin skin test (TST). A TST is the intradermal injection of a combination of mycobacterial antigens that elicit an immune response (delayed-type hypersensitivity), represented by induration, which can be measured in millimetres. The standard method of identifying people infected with M. tuberculosis is the TST using the Mantoux method. Multiple puncture tests should not be used as these tests are unreliable (because the amount of tuberculin injected intradermally cannot be precisely controlled). This annex describes how to administer, read and interpret a TST using 5 tuberculin units (TU) of tuberculin PPD-S. An alternative to 5 TU of tuberculin PPD-S is 2 TU of tuberculin PPD RT 23.
The results should be read between 48 and 72 hours after administration. A patient who does not return within 72 hours will probably need to be rescheduled for another TST.
Interpretation of TST depends on two factors: –diameter of the induration; –person's risk of being infected with TB and of progression to disease if infected. Induration of diameter ≥5 mm is considered positive in: HIV-positive children; –severely malnourished children (with clinical evidence of marasmus or kwashiorkor). Induration of diameter ≥10 mm is considered positive in: –all other children (whether or not they have received BCG vaccination). Causes of false-negative and false-positive TSTs are listed in Table A3.1. 1. Guidance for national tuberculosis programmes on the management of tuberculosis in children. Geneva: World Health Organization; 2006. (WHO/HTM/TB/2006.371) [PubMed: 17044200] |